Evaluation of Dye Removal Performance of Magnetic Dendrimers with Immobilized HRP Enzyme

Abstract

In this study, magnetic nanocomposites containing polyamidoamine (PAMAM) dendrimers were synthesized and horseradish peroxidase (HRP) immobilization was carried out on this support. Then, the potential use of immobilized HRP enzyme in the decolourization of Congo red and crystal violet dyes was investigated. To determine the optimum conditions, Congo Red and Crystal Violet removal percentages were determined in the presence of free and immobilized enzyme with various parameters such as pH (3-8), temperature (20-40 ℃), H₂O₂ concentration (1%-5%) and dye concentration (1.125-15 mg/L). When immobilized HRP was used, 99% decolourization was achieved for Crystal Violet after 25 minutes, while 75% decolourization was detected for Congo Red after 60 min. When we compared the immobilized and free enzyme for the decolourization of both dyes, the same removal was observed for Congo Red, while higher decolourization was observed for Crystal Violet when immobilized HRP was used.