Investigation of oxidative stress and some antioxidant in the tissues of female crayfish (Pontastacus leptodactylus) fed with trout diet

Abstract

The effects of trout and control diet on malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and reduced glutathione (GSH) levels in tissues (hepatopancreas, ovarian, muscle and gills) of Pontastacus leptodactylus was investigated. The control diet was formulated to contain approximately 37.0% crude protein, 7.6% crude fat. The trout diet was obtained from the feed factory as ready-made. The crude protein content of trout feed was 53.0%  and crude fat content was recorded as 15.0%. The experiment was carried out with 3 replicates for each dietary treatment. 15 females were used for each replicate (90 females in total). Statistically significant were detected that the levels of MDA increased in the hepatopancreas, ovarian, muscle and gills tissues of crayfish fed with trout diet according to control diet. In this study, SOD levels were found to be higher in trout diet group compared to control diet group. Furthermore, GSH levels in the trout diet groups were lower in hepatopancreas and ovarian, higher in muscle and gills than control diet group.

This study was planned to give a concrete answer to the question of whether crayfish can be grown with trout diet. At the end of the study, it was concluded that crayfish farming cannot be done with trout diet.